INFLUENCE OF COMPOSITION AND LYOPHILIZATION TIME ON PHYSICAL PROPERTIES OF HA/Cs/Coll/ HYDROXYPROPYL METHYLCELLULOSE BIOCOMPOSITES FOR BONE SCAFFOLDS

Dyah Purnaning; Hurnah Hurnah; Ahmad Taufik S; Susi Rahayu; Dian W. Kurniawidi; Kasnawi Al Hadi

doi:10.29303/ipr.v8i2.474

INFLUENCE OF COMPOSITION AND LYOPHILIZATION TIME ON PHYSICAL PROPERTIES OF HA/Cs/Coll/ HYDROXYPROPYL METHYLCELLULOSE BIOCOMPOSITES FOR BONE SCAFFOLDS

Authors

Dyah Purnaning , Hurnah Hurnah , Ahmad Taufik S , Susi Rahayu , Dian W. Kurniawidi , Kasnawi Al Hadi

DOI:

10.29303/ipr.v8i2.474

Published:

2025-05-30

Issue:

Vol. 8 No. 2 (2025)

Keywords:

Freeze Drying, Lyophilization, Strength

Articles

Downloads

PDF

How to Cite

Purnaning, D., Hurnah, H., Taufik S, A., Rahayu, S., Kurniawidi, D. W., & Hadi, K. A. (2025). INFLUENCE OF COMPOSITION AND LYOPHILIZATION TIME ON PHYSICAL PROPERTIES OF HA/Cs/Coll/ HYDROXYPROPYL METHYLCELLULOSE BIOCOMPOSITES FOR BONE SCAFFOLDS. Indonesian Physical Review, 8(2), 616–628. https://doi.org/10.29303/ipr.v8i2.474

Download Citation

Abstract

Biomaterial implants are one of the alternatives to replace damaged organs in the body system temporarily (scaffolding) or permanently. Bone biomaterial implants can be obtained through the manufacture of HA/Cs/Coll (HA/Cs/Coll) biocompositeses with the addition of Hydroxypropyl Methylcellulose (HPMC) matrix. The objective is to evaluate how variations in material composition and lyophilization time affect the physical properties of the biocomposites, including density, compressive strength, Young’s modulus, and surface morphology. The manufacture of biocomposites uses the mechanical thermal method for mixing materials and the freeze drying method for the biocomposites drying process. Composition ratios of HA:Cs/Coll were varied at 3:7, 5:5, and 7:3, while lyophilization durations were set at 24, 48, and 72 hours. Characterizations were performed through density measurements, mechanical testing using a Tensilon machine, and surface morphology analysis using a digital microscope. The results showed that the comparison of biocomposites with a ratio of 7:3 had the highest density of 0.150 gr/cm³, compressive strength of 0.046 MPa, and young modulus of 0.3 MPa. Meanwhile, the biocomposites that was lyophilized for 48 hours showed the best balance between a density of 0.145 gr/cm³, a compressive strength of 0.08 MPa, and a young modulus of 0.17 MPa. Morphological analysis revealed improved porosity and surface uniformity with longer freeze-drying times. Based on this, the resulting HA/Cs/Coll biocompositese has potential as an implant material but further research is needed to improve its mechanical properties by increasing the concentration of the binder, namely HPMC.

References

H. J. Sibarani, N. Olivia, And V. Syafrinanda, “Asuhan Keperawatan : Disfungsi Neurovaskuler Perifer Post Orif Dengan Tindakan Elevasi 200 Pada Ekstremitas Bawah Di Rumah Sakit Tk Ii Putri Hijau Medan,” Jurnal Riset Ilmiah, Vol. 2, Pp. 5014–5026, Dec. 2023.

Lembaga Penerbit Badan Penelitian Dan Pengembangan Kesehatan, “Laporan Nasional Riskesdas 2018,” 2019.

J. N. Setiadiputri, “Sintesis Dan Karakterisasi Biokomposit Hidroksiapatit-Alginat-Zinc Sebagai Bone Graft Untuk Penanganan Bone Defect. ,” J Chem Inf Model, Vol. 53, No. 9, Pp. 1689–1699, 2018.

H. , & F. Y. Fesseha, “Bone Grafting, Its Principle And Application,” A Review. Osteol Rheumatol Open J, Vol. 1, No. 1, Pp. 43–50, 2020.

T. P. E. Maharani, L. Rahmina, And S. Mutia, “Transplantasi Organ Tubuh Manusia Dalam Perspektif Etika Kedokteran Dan Agama Islam,” Jurnal Religion: Jurnal Agama, Sosial, Dan Budaya, Vol. 1, No. 4, Pp. 1184–1197, 2023.

M. D. Effendi And N. Suhendar, “Pengaruh Rasio Ha/Tcp Terhadap Karakteristik Komposit Bcp-Gelatin-Cmc Sebagai Bahan Injectable Bone Substitute,” Jurnal Inovasi Dan Teknologi Material, Vol. 1, No. 2, Pp. 15–20, 2019.

T. Tariverdian, F. Sefat, M. Gelinsky, And M. Mozafari, “Scaffold For Bone Tissue Engineering,” In Handbook Of Tissue Engineering Scaffolds: Volume One, Elsevier, Pp. 189–209, 2019.

T. , S. A. , E. M. H. , M. A. , M. J. , & M. A. Ghassemi, “Current Concepts In Scaffolding For Bone Tissue Engineering.,” Archives Of Bone And Joint Surgery, Vol. 6, No. 2, Pp. 90–99, 2018.

M. N. Collins, G. Ren, K. Young, S. Pina, R. L. Reis, And J. M. Oliveira, “Scaffold Fabrication Technologies And Structure/Function Properties In Bone Tissue Engineering,” Adv Funct Mater, Vol. 31, No. 21, Pp. 1–22, May 2021.

Z. Fereshteh, “Freeze-Drying Technologies For 3d Scaffold Engineering,” In Functional 3d Tissue Engineering Scaffolds: Materials, Technologies, And Applications, Elsevier, Pp. 151–174, 2018.

A. N. Banamtuan, G. M. Sipahelut, G. Ermiani, And M. Malelak, “Pengaruh Penggunaan Ekstrak Rosela (Hibiscus Sabdariffa Linn) Terhadap Kualitas Kimia Se’i Sapi (Effect Of Using Extract Roselle (Hibiscus Sabdariffa Linn) On Chemical Content Of Beef Se’i ),” Vol. 5, No. 1, Pp. 64–70, 2018.

Siswanto, D. Hikmawati, And M. Hariyanto, “Synthesis Of Hydroxyapatite Based On Coral Banyuwangi Using Sol-Gel Method: Observe The Effect Of Calcination Temperature On Its Phase And Crystallinity,” In Journal Of Physics: Conference Series, Institute Of Physics Publishing, Feb. 2019.

D. Supangat And S. E. Cahyaningrum, “Synthesis And Characterization Of Hydroxyapatite Of Crabs Shell (Scylla Serrata) By Wet Application Method,” 2017.

F. Kazemi-Aghdam, V. Jahed, M. Dehghan-Niri, F. Ganji, And E. Vasheghani-Farahani, “Injectable Chitosan Hydrogel Embedding Modified Halloysite Nanotubes For Bone Tissue Engineering,” Carbohydr Polym, Vol. 269, Oct. 2021.

P. Ma, W. Wu, Y. Wei, L. Ren, S. Lin, And J. Wu, “Biomimetic Gelatin/Chitosan/Polyvinyl Alcohol/Nano-Hydroxyapatite Scaffolds For Bone Tissue Engineering,” Mater Des, Vol. 207, Sep. 2021.

Anjarsari, K. Dahlan, P. Suptijah, And T. Kemala, “Sintesis Dan Karakterisasi Biokomposit Bcp/Kolagen Sebagai Material Perancah Tulang Synthesis And Characterization Of Biocomposite Bcp/Collagen For Bone Material Scaffold,” Jphpi, Vol. 19, No. 3, Pp. 356–361, 2016.

F. Xing Et Al., “Chitin-Hydroxyapatite-Collagen Composite Scaffolds For Bone Regeneration,” Int J Biol Macromol, Vol. 184, Pp. 170–180, Aug. 2021.

S. Rahayu, Masruroh, D. J. D. H. Santjojo, N. Septiani, And R. Wirawan, “Isolation And Characterization Of Chitosan From Nacre Of The Oyster Pearl Shell (Pinctada Maxima) As Biopolimer,” J Phys Conf Ser, Vol. 2980, No. 1, P. 012026, Apr. 2025.

M. Alaydrus Et Al., “Effectiveness Study Of Chitosan From Pearl Oyster Shells (Pinctada Maxima Sp.) As Antibacterial In Bone Scaffold Application,” Jurnal Biologi Tropis, Vol. 25, No. 1, Pp. 65–71, Jan. 2025.

E. F. Morgan, G. U. Unnikrisnan, And A. I. Hussein, “Bone Mechanical Properties In Healthy And Diseased States,” Annual Reviews Inc , Jun. 04, 2018.

H. D. , R. M. , E. S. , Dan M. A. Rahmayanti, “Karakterisasi Sifat Mekanik Benang Wol Dan Benang Kasur,” Jurnal Fisika, Vol. 9, No. 1, Pp. 28–36, 2019.

S. A. Cahyaningrum And N. Herdyastuti, “Pengembangan Biomaterial Kolagen Hidroksiapatit Kitosan Untuk Restorasi Jaringan Tulang (Bone Graft),” 2017.

H. Kenar, C. Y. Ozdogan, C. Dumlu, E. Doger, G. T. Kose, And V. Hasirci, “Microfibrous Scaffolds From Poly(L-Lactide-Co-Ε-Caprolactone) Blended With Xeno-Free Collagen/Hyaluronic Acid For Improvement Of Vascularization In Tissue Engineering Applications,” Materials Science And Engineering C, Vol. 97, Pp. 31–44, Apr. 2019.

S. L. Arifah And S. E. Cahyaningrum, “Sintesis Dan Karakterisasi Komposit Hidroksiapatit-Kitosan-Kolagen Sebagai Biomaterial Bone Graft Synthesis And Characteritation Of Hydroxyapatite-Chitosan-Collagen For Bone Graft Biomaterials,” 2017.

X. Song, M. A. Philpott, S. M. Best, And R. E. Cameron, “Controlling The Architecture Of Freeze-Dried Collagen Scaffolds With Ultrasound-Induced Nucleation,” Polymers (Basel), Vol. 16, No. 2, Jan. 2024.

C. Katrilaka Et Al., “Freeze-Drying Process For The Fabrication Of Collagen-Based Sponges As Medical Devices In Biomedical Engineering,” Mdpi, Jun. 01, 2023.

C. Dong And Y. Lv, “Application Of Collagen Scaffold In Tissue Engineering: Recent Advances And New Perspectives,” Mdpi Ag, 2016.

H. Zhao, L. Li, S. Ding, C. Liu, And J. Ai, “Effect Of Porous Structure And Pore Size On Mechanical Strength Of 3d-Printed Comby Scaffolds,” Mater Lett, Vol. 223, Pp. 21–24, Jul. 2018.

C. M. Murphy And F. J. O’brien, “Understanding The Effect Of Mean Pore Size On Cell Activity In Collagen-Glycosaminoglycan Scaffolds,” Taylor And Francis Inc, 2010.

M. Khoiriyah And S. E. Cahyaningrum, “Sintesis Dan Karakterisasi Bone Graft Dari Komposit Hidroksiapatit/Kolagen/Kitosan (Ha/Coll/Chi) Dengan Metode Ex-Situ Sebagai Kandidat Implan Tulang Synthesis And Characteritation Of Bone Graft From Hydroxyapatite/Collagen/Chitosan (Ha/Coll/Chi) Composite By Ex-Situ Method As A Bone Implant Candidates,” 2018.

K. M. Pawelec, A. Husmann, S. M. Best, And R. E. Cameron, “A Design Protocol For Tailoring Ice-Templated Scaffold Structure,” J R Soc Interface, Vol. 11, No. 92, Mar. 2014.

D. E. L. Angulo And P. J. Do Amaral Sobral, “The Effect Of Processing Parameters And Solid Concentration On The Microstructure And Pore Architecture Of Gelatin-Chitosan Scaffolds Produced By Freeze-Drying,” Materials Research, Vol. 19, No. 4, Pp. 839–845, Jul. 2016.

E. Capuana, F. Lopresti, F. Carfì Pavia, V. Brucato, And V. La Carrubba, “Solution-Based Processing For Scaffold Fabrication In Tissue Engineering Applications: A Brief Review,” Mdpi Ag, Jul. 01, 2021.

Y. Chen, T. T. Mutukuri, N. E. Wilson, And Q. (Tony) Zhou, “Pharmaceutical Protein Solids: Drying Technology, Solid-State Characterization And Stability,” Elsevier B.V, May 01, 2021.

Author Biographies

Dyah Purnaning, Medical Departement, Faculty of Medicine and Health Sciences, University of Mataram, Indonesia

Medicine Departement

Hurnah Hurnah, Physics Department, Faculty Mathematics and Natural Science University of Mataram, Indonesia

Physics Departemen

Susi Rahayu, Physics Department, Faculty Mathematics and Natural Science University of Mataram, Indonesia

Physics Departement

License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Authors who publish with Indonesian Physical Review Journal, agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International Licence (CC BY SA-4.0). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Indonesian Physical Review Journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

INFLUENCE OF COMPOSITION AND LYOPHILIZATION TIME ON PHYSICAL PROPERTIES OF HA/Cs/Coll/ HYDROXYPROPYL METHYLCELLULOSE BIOCOMPOSITES FOR BONE SCAFFOLDS

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Abstract

References

Author Biographies

Dyah Purnaning, Medical Departement, Faculty of Medicine and Health Sciences, University of Mataram, Indonesia

Hurnah Hurnah, Physics Department, Faculty Mathematics and Natural Science University of Mataram, Indonesia

Susi Rahayu, Physics Department, Faculty Mathematics and Natural Science University of Mataram, Indonesia

License

Official Website Of

Indonesian Physical Review, University of Mataram

Contact Us

Official link

Share & Follow