ANALYSIS OF HYDROXYAPATITE/GELATINE COMPOSITION ON THE FILAMENT FORMATION USING PISTON EXTRUSION METHOD

Yoga A Tandanu; Dionysius J D H Santjojo; Masruroh Masruroh

doi:10.29303/ipr.v7i1.273

ANALYSIS OF HYDROXYAPATITE/GELATINE COMPOSITION ON THE FILAMENT FORMATION USING PISTON EXTRUSION METHOD

Authors

Yoga A Tandanu , Dionysius J D H Santjojo , Masruroh Masruroh

DOI:

10.29303/ipr.v7i1.273

Published:

2024-01-29

Issue:

Vol. 7 No. 1 (2024)

Keywords:

Filaments, Viscosity, Hydroxyapatite, Gelatine, Extrusion Piston

Articles

Downloads

PDF

How to Cite

Tandanu, Y. A., Santjojo, D. J. D. H., & Masruroh, M. (2024). ANALYSIS OF HYDROXYAPATITE/GELATINE COMPOSITION ON THE FILAMENT FORMATION USING PISTON EXTRUSION METHOD. Indonesian Physical Review, 7(1), 153–165. https://doi.org/10.29303/ipr.v7i1.273

Download Citation

Abstract

HA/Gelatine bio composite is the main material in making scaffolds that have the advantage of biocompatibility and high biodegradability. One method of making scaffolds is using piston extrusion 3D printing technology, which is compatible with several types of materials, especially HA/gelatine biocomposites. This study aims to determine the effect of HA/gelatine bio composite composition on the filament formation process which is influenced by the rheological properties of the material. The filament extrusion process is influenced by rheological properties in the form of viscosity. The synthesized HA material was then dissolved with gelatin in the ratio of 1:2, 1:3, 1:4, 1:5, and 1:6 homogeneously. After that, viscosity measurements were made on each variation of HA/gelatine composition with a viscometer. The biocomposite solution that has been mixed homogeneously is then extracted until it comes out of the nozzle. Meanwhile, the viscosity of the HA/gelatine bio composite solution when given piston pressure can be known through the calculation process. The viscosity test results show that there is a change in the viscosity of the solution. This is caused by the shear-thickening phenomenon due to the application of pressure on the fluid. Based on the experimental results, the extrusion results still do not form filaments, which indicates that the rheological properties of the HA/gelatine bio composite solution are still too liquid so other material modifications are needed. The extrusion speed of 0.42 mm/s used in this study is too fast for the HA/gelatin material solution, so it has not formed optimal filaments.

References

M. Sari, P. Hening, Chotimah, I. D. Ana, and Y. Yusuf, “Bioceramic hydroxyapatite-based scaffold with a porous structure using honeycomb as a natural polymeric Porogen for bone tissue engineering,” Biomater Res, vol. 25, no. 1, pp. 1–13, Dec. 2021, doi: 10.1186/s40824-021-00203-z.

D. T. Dixon and C. T. Gomillion, “Conductive Scaffolds for Bone Tissue Engineering: Current State and Future Outlook,” JFB, vol. 13, no. 1, p. 1, Dec. 2021, doi: 10.3390/jfb13010001.

G. Fernandez De Grado et al., “Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management,” J Tissue Eng, vol. 9, p. 204173141877681, Jan. 2018, doi: 10.1177/2041731418776819.

S. Afewerki, A. Sheikhi, S. Kannan, S. Ahadian, and A. Khademhosseini, “Gelatin-polysaccharide composite scaffolds for 3D cell cultureand tissue engineering: Towards natural therapeutics,” Bioengineering & Translational Medicine, vol. 4, no. 1, pp. 96–115, Jan. 2018.

J. Visser et al., “Endochondral bone formation in gelatin methacrylamide hydrogel with embedded cartilage-derived matrix particles,” Biomaterials, vol. 37, pp. 174–182, Jan. 2015, doi: 10.1016/j.biomaterials.2014.10.020.

M. R. Nawafi, M. Masruroh, and D. J. D. H. Santjojo, “Morphological and Mechanical Study of Gelatin/Hydroxyapatite Composite based Scaffolds for Bone Tissue Regeneration,” Indonesian J Appl Phys, vol. 12, no. 2, p. 235, Nov. 2022, doi: 10.13057/ijap.v12i2.59365.

M. N. Collins, G. Ren, K. Young, S. Pina, R. L. Reis, and J. M. Oliveira, “Scaffold Fabrication Technologies and Structure/Function Properties in Bone Tissue Engineering,” Adv Funct Materials, vol. 31, no. 21, p. 2010609, May 2021, doi: 10.1002/adfm.202010609.

Hartatiek et al., “Nanostructure, porosity and tensile strength of PVA/Hydroxyapatite composite nanofiber for bone tissue engineering,” Materials Today: Proceedings, vol. 44, pp. 3203–3206, 2021, doi: 10.1016/j.matpr.2020.11.438.

C. Wang et al., “3D printing of bone tissue engineering scaffolds,” Bioactive Materials, vol. 5, no. 1, pp. 82–91, Mar. 2020, doi: 10.1016/j.bioactmat.2020.01.004.

B. Zhang, R. Cristescu, D. B. Chrisey, and R. J. Narayan, “Solvent-based Extrusion 3D Printing for the Fabrication of Tissue Engineering Scaffolds,” IJB, vol. 6, no. 1, p. 211, Jan. 2020, doi: 10.18063/ijb.v6i1.211.

Z. Fu, V. Angeline, and W. Sun, “Evaluation of Printing Parameters on 3D Extrusion Printing of Pluronic Hydrogels and Machine Learning Guided Parameter Recommendation,” IJB, vol. 7, no. 4, p. 434, Jan. 2021, doi: 10.18063/ijb.v7i4.434.

S. Jang et al., “Effect of material extrusion process parameters on filament geometry and inter-filament voids in as-fabricated high solids loaded polymer composites,” Additive Manufacturing, vol. 47, p. 102313, Nov. 2021, doi: 10.1016/j.addma.2021.102313.

H. Zhang, J. Wang, Y. Liu, X. Zhang, and Z. Zhao, “Effect of processing parameters on the printing quality of 3D printed composite cement-based materials,” Materials Letters, vol. 308, p. 131271, Feb. 2022, doi: 10.1016/j.matlet.2021.131271.

S. Syauqiyah, D. J. D. H. Santjojo, Masruroh, and H. A. Dharmawan, “The Design of a Piston Extruder for the Production of Gelatin Filaments Available for Hydroxyapatite Biocomposite 3D printing,” presented at the International Conference on Functional Materials Science, Bali: International Conference on Functional Materials Science, 2022, pp. 1–8.

R. Ershadnia et al., “Non-Newtonian fluid flow dynamics in rotating annular media: Physics-based and data-driven modeling,” Journal of Petroleum Science and Engineering, vol. 185, p. 106641, Feb. 2020, doi: 10.1016/j.petrol.2019.106641.

M. S. Salehi, M. T. Esfidani, H. Afshin, and B. Firoozabadi, “Experimental investigation and comparison of Newtonian and non-Newtonian shear-thinning drop formation,” Experimental Thermal and Fluid Science, vol. 94, pp. 148–158, Jun. 2018, doi: 10.1016/j.expthermflusci.2018.02.006.

N. Aldi et al., “Experimental and Numerical Analysis of a Non-Newtonian Fluids Processing Pump,” Energy Procedia, vol. 126, pp. 762–769, Sep. 2017, doi: 10.1016/j.egypro.2017.08.247.

B. Zhang et al., “Porous bioceramics produced by inkjet 3D printing: Effect of printing ink formulation on the ceramic macro and micro porous architectures control,” Composites Part B: Engineering, vol. 155, pp. 112–121, Dec. 2018, doi: 10.1016/j.compositesb.2018.08.047.

J. E. Trachtenberg, J. K. Placone, B. T. Smith, J. P. Fisher, and A. G. Mikos, “Extrusion-based 3D printing of poly(propylene fumarate) scaffolds with hydroxyapatite gradients,” Journal of Biomaterials Science, Polymer Edition, vol. 28, no. 6, pp. 532–554, Apr. 2017, doi: 10.1080/09205063.2017.1286184.

S. Brown, D. Montfort, N. Perova‐Mello, B. Lutz, A. Berger, and R. Streveler, “Framework Theory of Conceptual Change to Interpret Undergraduate Engineering Students’ Explanations About Mechanics of Materials Concepts,” J of Engineering Edu, vol. 107, no. 1, pp. 113–139, Jan. 2018, doi: 10.1002/jee.20186.

G. Zhong, M. Vaezi, P. Liu, L. Pan, and S. Yang, “Characterization approach on the extrusion process of bioceramics for the 3D printing of bone tissue engineering scaffolds,” Ceramics International, vol. 43, no. 16, pp. 13860–13868, Nov. 2017, doi: 10.1016/j.ceramint.2017.07.109.

M. Vaezi, G. Zhong, H. Kalami, and S. Yang, “Extrusion-based 3D printing technologies for 3D scaffold engineering,” in Functional 3D Tissue Engineering Scaffolds, Elsevier, 2018, pp. 235–254. doi: 10.1016/B978-0-08-100979-6.00010-0.

B. Munson, D. Young, T. Okiishi, and W. Huebsch, Fundamental of Fluids Mechanics, Sixth Edition. United States of America: John Wiley & Sons, Inc., 2016.

P. Geng et al., “Effects of extrusion speed and printing speed on the 3D printing stability of extruded PEEK filament,” Journal of Manufacturing Processes, vol. 37, pp. 266–273, Jan. 2019, doi: 10.1016/j.jmapro.2018.11.023.

M. Hassan, “Thermal energy and mass transport of shear thinning fluid under effects of low to high shear rate viscosity,” 2022.

H. Herrada-Manchón, D. Rodríguez-González, M. A. Fernández, N. W. Kucko, F. Barrère-de Groot, and E. Aguilar, “Effect on Rheological Properties and 3D Printability of Biphasic Calcium Phosphate Microporous Particles in Hydrocolloid-Based Hydrogels,” Gels, vol. 8, no. 1, p. 28, Jan. 2022, doi: 10.3390/gels8010028.

R. B. Islami, L. A. Didik, and B. Bahtiar, “Determine of the nira water viscosity by using video based laboratory falling ball method with tracker software,” Gravity Untirta, vol. 7, no. 2, Aug. 2021, doi: 10.30870/gravity.v7i2.10165.

L. C. Sow and H. Yang, “Effects of salt and sugar addition on the physicochemical properties and nanostructure of fish gelatin,” Food Hydrocolloids, vol. 45, pp. 72–82, Mar. 2015, doi: 10.1016/j.foodhyd.2014.10.021.

M. Cutini, M. Corno, D. Costa, and P. Ugliengo, “How Does Collagen Adsorb on Hydroxyapatite? Insights From Ab Initio Simulations on a Polyproline Type II Model,” J. Phys. Chem. C, vol. 123, no. 13, pp. 7540–7550, Apr. 2019, doi: 10.1021/acs.jpcc.7b10013.

Siswanto Siswanto, D. Hikmawati, U. Kulsum, D. I. Rudyardjo, R. Apsari, and Aminatun Aminatun, “Biocompatibility and osteoconductivity of scaffold porous composite collagen–hydroxyapatite based coral for bone regeneration,” Open Chemistry, vol. 18, no. 1, pp. 584–590, Jun. 2020, doi: 10.1515/chem-2020-0080.

F. J. García García and P. Fariñas Alvariño, “On an analytic solution for general unsteady/transient turbulent pipe flow and starting turbulent flow,” European Journal of Mechanics - B/Fluids, vol. 74, pp. 200–210, Mar. 2019, doi: 10.1016/j.euromechflu.2018.11.014.

Z. Wu, L. Zeng, K. Chen, J. Chen, and Y. Zhang, “Experiments on Laminar Flow between Parallel Plates with a Heterogeneous Slip/No-Slip Surface,” Tribology Transactions, vol. 62, no. 5, pp. 801–811, Sep. 2019, doi: 10.1080/10402004.2019.1619005.

N. P. Kim, J.-S. Eo, and D. Cho, “Optimization of piston type extrusion (PTE) techniques for 3D printed food,” Journal of Food Engineering, vol. 235, pp. 41–49, Oct. 2018, doi: 10.1016/j.jfoodeng.2018.04.019.

P. Dee, S. Tan, and H. L. Ferrand, “Fabrication of Microstructured Calcium Phosphate Ceramics Scaffolds by Material Extrusion-Based 3D Printing Approach,” IJB, vol. 8, no. 2, p. 551, Feb. 2022, doi: 10.18063/ijb.v8i2.551.

L. Deng, Y. Li, A. Zhang, and H. Zhang, “Nano-hydroxyapatite incorporated gelatin/zein nanofibrous membranes: Fabrication, characterization and copper adsorption,” International Journal of Biological Macromolecules, vol. 154, pp. 1478–1489, Jul. 2020, doi: 10.1016/j.ijbiomac.2019.11.029.

Y. B. Pottathara, T. Vuherer, U. Maver, and V. Kokol, “Morphological, mechanical, and in-vitro bioactivity of gelatine/collagen/hydroxyapatite based scaffolds prepared by unidirectional freeze-casting,” Polymer Testing, vol. 102, p. 107308, Oct. 2021, doi: 10.1016/j.polymertesting.2021.107308.

M. E. Rosti and S. Takagi, “Shear-thinning and shear-thickening emulsions in shear flows,” Physics of Fluids, vol. 33, no. 8, p. 083319, Aug. 2021, doi: 10.1063/5.0063180.

T. Shende, V. J. Niasar, and M. Babaei, “An empirical equation for shear viscosity of shear thickening fluids,” Journal of Molecular Liquids, vol. 325, p. 115220, Mar. 2021, doi: 10.1016/j.molliq.2020.115220.

X. Chen, D. Wu, J. Xu, T. Yan, and Q. Chen, “Gelatin/Gelatin-modified nano hydroxyapatite composite scaffolds with hollow channel arrays prepared by extrusion molding for bone tissue engineering,” Mater. Res. Express, vol. 8, no. 1, p. 015027, Jan. 2021, doi: 10.1088/2053-1591/abde1f.

L. Wang, M. Li, X. Li, J. Liu, Y. Mao, and K. Tang, “A Biomimetic Hybrid Hydrogel Based on the Interactions between Amino Hydroxyapatite and Gelatin/Gellan Gum,” Macro Materials & Eng, vol. 305, no. 9, p. 2000188, Sep. 2020, doi: 10.1002/mame.202000188.

R. Baptista, M. Guedes, M. F. C. Pereira, A. Maurício, H. Carrelo, and T. Cidade, “On the effect of design and fabrication parameters on mechanical performance of 3D printed PLA scaffolds,” Bioprinting, vol. 20, p. e00096, Dec. 2020, doi: 10.1016/j.bprint.2020.e00096.

N. Reddy, R. Reddy, and Q. Jiang, “Crosslinking biopolymers for biomedical applications,” Trends in Biotechnology, vol. 33, no. 6, pp. 362–369, Jun. 2015, doi: 10.1016/j.tibtech.2015.03.008.

A. Lamp, M. Kaltschmitt, and J. Dethloff, “Options to Improve the Mechanical Properties of Protein-Based Materials,” Molecules, vol. 27, no. 2, p. 446, Jan. 2022, doi: 10.3390/molecules27020446.

C. E. Campiglio, N. Contessi Negrini, S. Farè, and L. Draghi, “Cross-Linking Strategies for Electrospun Gelatin Scaffolds,” Materials, vol. 12, no. 15, p. 2476, Aug. 2019, doi: 10.3390/ma12152476.

S. Glukhova et al., “Printable Alginate Hydrogels with Embedded Network of Halloysite Nanotubes: Effect of Polymer Cross-Linking on Rheological Properties and Microstructure,” Polymers, vol. 13, no. 23, p. 4130, Nov. 2021, doi: 10.3390/polym13234130.

D. J. Choi, Y. Kho, S. J. Park, Y.-J. Kim, S. Chung, and C.-H. Kim, “Effect of cross-linking on the dimensional stability and biocompatibility of a tailored 3D-bioprinted gelatin scaffold,” International Journal of Biological Macromolecules, vol. 135, pp. 659–667, Aug. 2019, doi: 10.1016/j.ijbiomac.2019.05.207.

License

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Authors who publish with Indonesian Physical Review Journal, agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International Licence (CC BY SA-4.0). This license allows authors to use all articles, data sets, graphics, and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Indonesian Physical Review Journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

ANALYSIS OF HYDROXYAPATITE/GELATINE COMPOSITION ON THE FILAMENT FORMATION USING PISTON EXTRUSION METHOD

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Abstract

References

License

Official Website Of

Indonesian Physical Review, University of Mataram

Contact Us

Official link

Share & Follow